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APPLICATION NOTE:
Several researchers use our PEEK MicroTee to introduce ionizing voltage to their fluid stream just prior to their Mass Spectrometer1. The P-775 and P-875 MicroTees are well suited for this application due to their advantageous internal geometry and PEEK polymer's electrical resistance. The materials required for this setup are as follows: one gold or platinum conducting wire, one P-775 or P-875 MicroTee (this page), one MicroTight Tubing Sleeve (page 29) for the conducting wire (as needed to accomodate wire diameter), and at least two more MicroTight Tubing Sleeves (page 29) to connect your capillary tubing. Connect the wire to the center port of the MicroTee. First, thread your wire through the appropriate tubing sleeve, if necessary, with the wire extending beyond both ends of the tubing sleeve. Slip the female nut included with the MicroTee over the wire or sleeved wire, followed by the ferrule, ensuring the wire (and its sleeve) extends well past the end of the ferrule tip. Align the tip of the wire with the thru-hole of the MicroTee and gently insert the wire until it bottoms out. Now finger tighten the female nut into place. Attach your flow path tubing to the MicroTee's two other available ports, following the instructions provided with the MicroTee. Begin fluid flow through the tee and apply voltage to the conducting wire lead. This setup typically provides effective electrospray ionization in applications having a flow rate of 100µL/min or greater.